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swoH1 mutant cells swell and lyse at restrictive temperature. Conidia of the swoH1 mutant AXL20 were inoculated onto coverslips in complete liquid medium and incubated at the restrictive temperature (42°C). The cells were fixed and stained with Hoechst 33258 to visualize the nuclei. The upper and lower rows show differential interference contrast (DIC) and fluorescent images of the same fields. (a) 6 h, (b) 8 h, (c) 10 h, (d) 12 h, (e) 24 h, (f) 38 h. Bar, 5 μm.
