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Subcellular localization of full-length or truncated Sla1-GFP proteins by live observation of cells. GFP fusion proteins were expressed under control of the nmt1 promoter in the vector pSLF172L-GFP. h90 wild-type cells (SP870) carrying each plasmid were grown in PM (+N) medium and incubated at 25°C. Cells were examined for GFP fluorescence, and the same cells were stained with the DNA-binding dye Hoechst 33342. Images of the cells are depicted on the right.
