Fig. 3.

Labeling pattern of a mixture of four rNMPs isolated from K10zwf, K12, and DL323 E. coli grown on a [3-¹³C]-Pyruvate background, and DL323 E. coli grown on a [1, 3-¹³C]-glycerol background. The direct carbon detection 1D spectrum shows all the labeled carbon positions. A long recycle delay of 5 s was used to allow for sufficient magnetization recovery and proton decoupling was limited to the acquisition period only. The residual ¹³C–¹³C coupling observed in a [1, 3-¹³C]-glycerol is absent with the [3-¹³C]-Pyruvate only in DL323; K10 and K12 grown on [3-¹³C]-Pyruvate still retain the residual ¹³C–¹³C coupling