Table 1.
13C enrichment levels at various carbon positions within ribonucleotides harvested from K12, DL323, and K10zwf E. coli strains grown on [3-13C]-pyruvate
| Carbon position labeled | DL323 E. coli strain | K10zwf E. coli strain | K12 E. coli strain |
|---|---|---|---|
| Purinea | |||
| Ade C2 | 99 ± 1 | 85 ± 4 | 95 ± 2 |
| C8 | 93 ± 1 | 57 ± 1 | 98 ± 2 |
| Pyrimidinea | |||
| C5 | 98 ± 4 | 81 ± 4 | 91 ± 1 |
| C6 | 15 ± 1 | 60 ± 4 | 64 ± 1 |
| Ribose | |||
| C1′a | 42 ± 4 | 82 ± 2 | 83 ± 2 |
| C2′b | 4 | 9 ± 1 | 21 ± 1 |
| C3′b | <2 | <1 | 8 |
| C4′b | 3 | 9 ± 1 | 30 ± 1 |
| C5′c | 95 ± 5 | 95 | 95 ± 1 |
aThe percentage label is calculated as an average of two methods: (i) the ratio of the sum of the intensities of satellite peaks to the sum of the intensities of the satellite and center peaks using the 2-bond 15N HSQC without 13C decoupling during acquisition (Dayie and Thakur 2010) and (ii) the ratio of the sum of the intensities of satellite peaks to the sum of the intensities of the satellite and center peaks using the 1D 1H experiment without 13C decoupling during acquisition as described in the text
b,cThe percentage label (Plabel) is calculated as in (a) but this time with only method (ii)