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. 2003 Dec;2(6):1246–1252. doi: 10.1128/EC.2.6.1246-1252.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 6. — Activation of ena1 transcription requires high salt concentrations, alkaline ambient pH, and PacC. Transcript levels of ena1 in the F. oxysporum wild-type strain, pacC^+/− mutant, and pacC^c mutant were analyzed by northern hybridization analysis. (A) Microconidia of the wild-type strain were germinated for 12 h in SM without added Na⁺ and then transferred for 2 h to SM buffered at the indicated pH values, with or without 0.5 M Na⁺. Total RNA was extracted, fractionated on an agarose gel, blotted onto a nylon membrane and hybridized with the ena1 probe. As a loading control, a probe corresponding to the F. oxysporum actin gene (act1) was hybridized to the same RNA samples. (B) Microconidia of the wild-type strain, the pacC^+/− mutant and the pacC^c mutant were germinated as in panel A and transferred to SM buffered at pH 8.0 and containing 0.5 M Na⁺. Total RNA was extracted from samples obtained at the indicated time points, fractionated, blotted, and hybridized with the ena1 or act1 probes.

FIG. 6. — Activation of ena1 transcription requires high salt concentrations, alkaline ambient pH, and PacC. Transcript levels of ena1 in the F. oxysporum wild-type strain, pacC^+/− mutant, and pacC^c mutant were analyzed by northern hybridization analysis. (A) Microconidia of the wild-type strain were germinated for 12 h in SM without added Na⁺ and then transferred for 2 h to SM buffered at the indicated pH values, with or without 0.5 M Na⁺. Total RNA was extracted, fractionated on an agarose gel, blotted onto a nylon membrane and hybridized with the ena1 probe. As a loading control, a probe corresponding to the F. oxysporum actin gene (act1) was hybridized to the same RNA samples. (B) Microconidia of the wild-type strain, the pacC^+/− mutant and the pacC^c mutant were germinated as in panel A and transferred to SM buffered at pH 8.0 and containing 0.5 M Na⁺. Total RNA was extracted from samples obtained at the indicated time points, fractionated, blotted, and hybridized with the ena1 or act1 probes.