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. Author manuscript; available in PMC: 2013 Jan 20.
Published in final edited form as: Cell. 2012 Jan 20;148(1-2):349–361. doi: 10.1016/j.cell.2011.11.025

Figure 2. EMT precedes tumor formation.

Figure 2

(A–D) In pancreata taken from 8–10 wk-old PKCY mice, EMT is observed in regions of acinarto-ductal metaplasia with inflammation (ADMI; D), PanIN 2 (B), PanIN 3 (C), but not in PanIN 1 lesions (A). Arrows show individual YFP+ cells (green) that also express Zeb1 (red). (E) Quantification of observations from A–D, showing the percentage of each type of lesion having at least one cell that has undergone EMT; numbers reflect at least 10 medium-powered fields from each of five PanIN mice. (F) Strategy for isolating YFP+ epithelially-derived cells from the pancreas; the purity of the YFP+ population was confirmed by a repeat FACS analysis. (G) Transcriptional analysis of sorted YFP+ pancreas cells from lineage-labeled CY control (n=4), PanIN (n=6), and PDAC (n=5) pancreata. Bar graph data are presented as mean ± SD in this and subsequent figures. *, p<0.01; **, p<0.001 by two-tailed Student's t-test in this and subsequent figures, unless otherwise noted. Scale bars, 20μm. See also Figure S2.