TABLE 3.
Annotated SAGE tags for the top 100 tags that are unique to the in vivo library relative to in vitro
| Tag | No. of tags detected in vivo | BLAST result | Species | E value | % Identity | % Similarity | GO biological process | |
|---|---|---|---|---|---|---|---|---|
| TGATTATGAA | 25 | Glutamine-dependent NAD+ synthase | Saccharomyces cerevisiae | 1.0E-116 | 50 | 59 | Amino acid metabolism | |
| CATATTATGT | 22 | Hypothetical protein | Neurospora crassa | 1.0E-24 | 46 | 75 | Uncharacterized | |
| TATCTATGTA | 18 | Receptor-associated protein | Schizosaccharomyces pombe | 1.0E-13 | 40 | 62 | Antiapoptosis | |
| CATTTGTATA | 17 | G10 protein homolog | Pleurotus ostreatus | 2.0E-42 | 85 | 96 | mRNA splicing | |
| CCAATGATTA | 17 | Aconitase protein | Bacteroides fragilis | 3.0E-06 | 50 | 75 | Carbohydrate metabolism | |
| GACATATGAA | 17 | Alpha-amylase | Debaryomyces occidentalis | 1.0E-21 | 33 | 55 | Carbohydrate metabolism | |
| TGTTATATGA | 17 | Inositol monophosphatase | Rattus norvegicus | 1.0E-37 | 39-44 | 59-61 | Carbohydrate metabolism | |
| GAGAGTGTTA | 16 | VHS domain protein | S. pombe | 5.0E-17 | 33 | 52 | Vesicle transport | |
| ATTAGTTAAT | 15 | Clathrin coat assembly protein | S. pombe | 5.0E-19 | 40-53 (3)a | 58-79 (3) | Vesicle transport | |
| TAACATACAT | 15 | Oligosaccharyltransferase subunit | Mus musculus | 3.0E-05 | 31-63 (3) | 41-72 (3) | Antiapoptosis | |
| ATTATAACGA | 14 | Proteasome component PUP2 | S. pombe | 2.0E-27 | 60-95 (2) | 74-95 (2) | Protein catabolism | |
| ATTCACAGGA | 14 | Regulatory particle non-ATPase; Rpn9p | S. cerevisiae | 8.0E-21 | 46 | 68 | Protein catabolism | |
| CATATTATGT | 14 | Amino acid transporter | Uromyces viciae fabae | 1.0E-119 | 38-64 (3) | 61-78 (3) | Transport | |
| CATCTTAGAT | 13 | UMP-CMP kinase | Lentinula edodes | 7.0E-50 | 65 | 80 | Signal transduction | |
| TATTCTACAC | 13 | Predicted protein | N. crassa | 8.0E-12 | 45 | 57 | Uncharacterized | |
| ATTTTCAGAA | 12 | Dynamin-related protein | S. pombe | 3.0E-16 | 50 | 69 | Mitochondrial organization-biogenesis | |
| CATAGAATTA | 12 | RNA polymerase II subunit RPB9 | S. cerevisiae | 4.0E-07 | 48 | 63 | Transcription | |
| GAATTGATAG | 12 | Arc1p | S. cerevisiae | 2.0E-18 | 44 | 58 | Amino acid metabolism | |
| TACTTCTTGA | 12 | Related to BET1 protein | N. crassa | 1.0E-09 | 34 | 52 | Vesicle transport | |
| AATTATTATT | 11 | Hypothetical protein | S. pombe | 2.0E-06 | 24 | 45 | Uncharacterized | |
| GGTCAGTCGA | 11 | Hypothetical protein | Plasmodium yoelii yoelii | 1.0E-17 | 72-77 (2) | 85-89 (2) | Uncharacterized | |
| TATTTCATTT | 11 | U3 small nucleolar RNP protein IMP3 | S. cerevisiae | 9.0E-50 | 59 | 74 | RNA splicing | |
| TTATGGGTAG | 11 | Bud20p | S. cerevisiae | 7.0E-11 | 44 | 57 | Nuclear pore | |
| AATGTATTAA | 10 | Stomatin-like protein | Gibberella fujikuroi | 2.0E-13 | 57-63 (2) | 72-79 (2) | Cell wall maintenance | |
| CAATCAATAT | 10 | FKBP-70; immunophilin | S. cerevisiae | 9.0E-39 | 70 | 81 | Protein biosynthesis | |
| CATAGATGCC | 10 | Hypothetical protein | N. crassa | 5.0E-11 | 35 | 54 | Uncharacterized | |
| CATTGTTACT | 10 | NADH/ubiquinone oxidoreductase | N. crassa | 2.0E-45 | 63 | 78 | Cellular respiration | |
| CATTTTGACT | 10 | Ribosomal protein L27 | Filobasidium floriforme | 5.0E-77 | 84 | 85 | Protein biosynthesis | |
| TTTATAGATC | 10 | Mitochondrial carrier | N. crassa | 3.0E-53 | 67 | 78 | Mitochondrial organization-biogenesis |
a
Values in parentheses refer to the number of BLAST hits within the proteins that were divided by introns.