Skip to main content
. Author manuscript; available in PMC: 2013 Jan 20.
Published in final edited form as: Cell. 2012 Jan 20;148(1-2):164–174. doi: 10.1016/j.cell.2011.11.023

Figure 2. CFTR Inter-Residue Coupling Matrix Calculated from an ABC Transporter Sequence Alignment.

Figure 2

(A) (left) Heat map of the 1480 × 1480 matrix representing a linear combination of statistical methods (SCA, ELSC, OMES, McBASC). The color scale (right) with statistical scores approaching zero represented by dark blue and scores of high statistical significance in red. The diagonal denotes identity. Coupling of each of the five domains of CFTR to NBD1 are shown in squares with TMD1 in orange, NBD1 coupling to itself in light gray, the R-region in purple, TMD2 in pink, and NBD2 in dark gray. See also Figure S2. (right) The 285 × 285 matrix of coupling scores within NBD1 (positions 389 to 673 of the left panel). Above the matrix is the column of coupling scores for every position in NBD1 to the 508 position. The conserved Walker A and B, Consensus motifs, and the 508 position are denoted by arrows. (B) (left) The 508-coupling scores in ICL1 (residues 158-184) and ICL4 (residues 1050-1080) of the CFTR model (Figure 1B). The surface corresponding to the F508 position is colored in green and is predicted to be in contact with ICL4. (right) View of NBD1 (pdb 1R0W) colored with normalized scores from panel C. The F508 position is shown in green.