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. Author manuscript; available in PMC: 2013 Jan 19.
Published in final edited form as: Cell Host Microbe. 2012 Jan 19;11(1):81–90. doi: 10.1016/j.chom.2011.11.012

Figure 3.

Figure 3

The proapoptotic ΔnuoG mutant is present as fewer bacteria per infected cell than wild-type H37Rv. C57BL/6J mice were infected with GFP-expressing wild-type H37Rv or ΔnuoG M. tuberculosis, and GFP+ neutrophils, myeloid dendritic cells, alveolar macrophages, and recruited macrophages were sorted by FACS on day 17 post-infection. Lung cells from three mice were pooled prior to sorting, and three pools per group were analyzed, to ensure that 200–400 infected cells per pool could be examined by fluorescent microscopy. Cells containing 8 or more bacteria/cell are designated as 8+. Insufficient numbers of infected monocytes were obtained for analysis. Data are mean ± SD from 9 mice per group analyzed in pools of three mice per pool. *, p < 0.05; **, p < 0.01; ***, p < 0.001.