Table 1.
Summary of sequence reads obtained from different RNA-Seq libraries
| Library | Total reads | Mapped reads | Unmapped reads | % mapped reads |
|---|---|---|---|---|
| R_BD1 | 13,456,136 | 4,557,242 | 8,898,894 | 33,86% |
| ET_ BD 1 | 14,505,855 | 5,801,368 | 8,704,487 | 39,99% |
| LT_ BD 1 | 14,599,502 | 7,567,175 | 7,032,327 | 51,83% |
| Sc_ BD 1 | 9,861,818 | 1,487,604 | 8,374,214 | 15,08% |
| GII_ BD 1 | 9,294,474 | 3,647,906 | 5,646,568 | 39.25% |
| GV_ BD 1 | 10,641,921 | 6,610,820 | 4,031,101 | 62.12% |
| Oo_ BD 2 | 55,140,294 | 714,769 | 54,425,525 | 1.3% |
| LT_SS3+ | 13,928,058 | 8,852,802 | 5,075,256 | 63.56% |
| Sc_ SS 3+ | 14,360,448 | 8,386,042 | 5,974,406 | 58.40% |
| GII_ SS3+ | 9,331,268 | 4,952,677 | 4,378,591 | 53.08% |
| GV_ SS3+ | 20,197,230 | 6,534,612 | 13,662,618 | 32.35% |
R, ET, LT, Sc, GII, GV, and Oo correspond to ring, early trophozoite, late trophozoite, schizont, gametocyte II, gametocyte V, and ookinete stages, respectively. BD represents bidirectional libraries, and SS represents strand-specific libraries. "Total reads" corresponds to the initial output of sequencing after analysis with Illumina pipeline v3.1; "mapped reads" refers to numbers of reads mapped to the Pf3D7 reference genome (PlasmoDB version 7.1) allowing one single hit and no mismatches (1), one mismatch (2), or up to two mismatches (3) at the reads. + indicates the total numbers of reads obtained after de-multiplexing.