Fig. 3.
The presence of cardiac neural crest cell derivatives is reduced in the developing OFT in mice with gain or loss of Notch signaling in the NCC associated with congenital cardiac malformations. Expression of β-gal from the R26R allele was detected by blue X-gal staining of isolated hearts. Wnt1Cre;R26R;RosaNotch (B,E) and Wnt1Cre;R26R;RBP-Jf/f (C,F) embryos have reduced presence of neural crest-derived cells in the cardiac outflow tract and aortic valves at E14.5, as compared to Wnt1Cre;R26R controls (A, D, arrows, arrowheads). Dashed lines outline the outflow tract. PTA, persistent truncus arteriosus; DORV, double outlet right ventricle; AoV, aortic valve. (n=6 for each genotype at each age analyzed)