TABLE 1.
Summary of BDA Injections
| Injection area (mm2) |
Injection size category |
Labeled cell body profiles1 (no. per section) |
Corrected labeled cell bodies1 (no. per section) |
Labeled voxels1 (no. per section) |
Movement representations within the injection core
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Undefined (%) |
No response (%) |
Face (%) |
Proximal (%) |
Distal forelimb (%)
|
||||||||
| Wrist/ forearm |
Digits | Total | ||||||||||
| 1934 | 3.3 | Large | 51 | 38.2 | 314.8 | 0 | 1.2 | 0 | 13.5 | 68.5 | 16.8 | 85.3 |
| 1892 (BDA) | 1.92 | Large | 0.2 | 0.2 | 61.5 | 0 | 0 | 0 | 32.1 | 5.7 | 62.2 | 67.9 |
| 1892 (FB) | 3.31 | Large | 343.3 | 257.5 | N/A | 0 | 0 | 7.8 | 51.0 | 12.4 | 28.7 | 41.1 |
| 9409 | 0.9 | Small | 1.9 | 1.4 | 130.6 | 0 | 0 | 0 | 16.2 | 13.7 | 70.1 | 83.8 |
| 3024 | 0.8 | Small | 1.4 | 1.0 | 19.4 | 0 | 11.9 | 0 | 0 | 54.8 | 33.3 | 88.1 |
| 396 | 5.22 | Large | 44.2 | 33.2 | 2303.6 | 6.33 | 20.1 | 12.3 | 1.9 | 15.5 | 43.9 | 12.3 |
Note that numbers of labeled cells (or labeled voxels) per section are reported. Estimates of cell body numbers were corrected for overcounting (Abercrombie, 1946). For an approximation of the total number of labeled cells in the entire hemisphere, the number of cells per section must be multiplied by the total number of sections through the cortical gray matter. It should be kept in mind that these are general estimates since unbiased stereological techniques would be required for appropriate determination of actual numbers. The present data is appropriate, however, for estimates of proportions in each area. Using these methods of approximation, in only three cases the total no. BDA-labeled cell bodies in the contralateral hemisphere was estimated to be higher than one thousand prior to the Abercrombie’s correction factor (case 1934 = 1377; case 1892 FB = 9955; case 396 = 1238). Thus, only these cases were used for analysis of the distribution of cell bodies (cell count approximation with the global correction factor of 0.75: case 1934 = 1033; case 1892 = 7466; case 396 = 928).
These numbers are approximate in this case because the center of FB dense core of the injection was absent. This missing portion was considered to be fully contained in the core of the injection site of both FB and BDA (see Materials and Methods).
Because of the craniectomy border, there was a small proportion of these injection cores that was not physiologically defined. However, this area was most likely included in the distal forelimb area (see Figure 1).