Table 2.
Sample | τ (ns) | Θ (ns) |
---|---|---|
EAW10:OAW10 gel | 0.222± 0.02 (32.6%) | |
1.252 (73.4 %) 4.031 (26.6 %) |
0.389± 0.03 (33.1%) | |
> 50 (34.3%) | ||
| ||
EAW14:OAW14 gel | 0.248 ± 0.02 (38.0%) | |
1.237 (69.8 %) 3.967 (30.2 %) |
0.563 ± 0.03 (45.1%) | |
> 50 (16.9%) | ||
| ||
OAW10 Solution | 0.532 ± 0.04 (44.5%) | |
0.881 (55.1 %) 1.606 (44.9 %) |
2.243 ± 0.20 (52.0%) | |
11 ± 3.6 (3.5%) | ||
| ||
EAW10 Solution | 0.633 ± 0.04 (43.8%) | |
0.932 (56.8 %) 1.847 (43.2 %) |
2.287 ± 0.2 (52.9%) | |
11 ± 3.6 (3.3%) | ||
| ||
OAW14 Solution | 0.425 ± 0.03 (73.4%) | |
0.939 (43.3 %) 1.678 (56.7 %) |
6.847 ± 0.3 (8.3%) | |
12 ± 5.2 (18.3%) | ||
| ||
EAW14 Solution | 0.466 ± 0.03 (63.5%) | |
1.010 (47.8 %) 1.947 (52.2 %) |
11.8 ± 5.2 (20.9%) | |
22 ± 7.5 (15.6%) |
All lifetime measurements were performed at 25°C; τ is the average lifetime of the tryptophan fluorescence; Θ is the rotational correlation time from time-resolved anisotropy measurements. In gels, the component with the longest Θ value (> 50 ns) is very poorly defined because the fluorescence lifetime τ is much shorter than 50 ns. The percentage numbers in the parentheses mean the relative contributions of each individual component.