Table 2.
Samples | keff (mM−1·s−1)a | Cmobile (mM)b | Cimmobile (mM)c | R2 |
---|---|---|---|---|
5°C gel | 0.8 | 2.5 | 7.5 | 0.971 |
5°C → 25°C gel | -- | 0.5 | 9.5 | -- |
25°C gel | 24.5 | 0.4 | 9.6 | 0.999 |
25°C → 5°C gel | -- | 0.4 | 9.6 | -- |
For the 5°C gel and the 25°C gel, fitting Eqn. (3) gives keff and Cmobile. R2 is the goodness of fitting.
For the 5°C → 25°C gel and the 25° → 5°C gel, Cmobile is the last C(t) value collected from each gel.
Cimmobile = 10 mM – Cmobile in all cases.