Skip to main content
. 2012 Jan 3;122(2):722–732. doi: 10.1172/JCI58618

Figure 6. Decreased TGF-β1 production in the skin of ZD mice.

Figure 6

(A and B) Samples of (A) skin tissue or (B) mucosal tissue were taken from the backs of mice fed a ZA (white bars) or ZD (black bars) diet for 5 weeks and cultured for 24 hours (n = 5). TGF-β1 secretion in the ex vivo skin or mucosa organ cultures was assessed by ELISA assays. (C) SEAP activity of the skin samples measured using MFB-F11 cells with or without HCl in the culture system. (D) Quantitative real-time RT-PCR for the detection of Tgfb1 and Gapdh mRNAs in epidermis from the ears of mice fed a ZA (white bar) or ZD (black bar) diet for 5 weeks. The fold induction was calculated from normalized mRNA expression by the epidermis of ZD mice relative to that of ZA mice. Results are the mean ± SD (n = 5). *P < 0.05, compared with mice fed the ZA diet. Data are representative of several experiments with similar results.