Figure 11. Enhanced nephrin and Cas phosphorylation after protamine sulfate–induced foot process spreading.

WT mice were perfused with either buffer or protamine sulfate (PS), and paraffin-embedded kidney sections were stained with either anti–p-nephrin (red) or anti–p-Cas (red) and anti–ZO-1 (green) antibodies. Cas and nephrin phosphorylation was enhanced after protamine sulfate–induced podocyte injury. Original magnification, ×200.