Figure 14. Enhanced Cas and FAK phosphorylation in membranous nephropathy and minimal change disease.
(A) Representative images showing anti–p-Cas (green) and anti–p-FAK (green) antibody staining of frozen kidney sections from control subjects and from patients with focal segmental glomerulosclerosis (FSGS), membranous nephropathy (MN), or minimal change disease (MCD). (B) Coimmunofluorescence study of frozen kidney sections of patients with minimal change disease with anti–p-Cas (green) or anti–p-FAK antibody (green) and the podocyte slit diaphragm marker synaptopodin (red). Higher-magnification images are shown below. Note that p-Cas and p-FAK almost completely colocalized with synaptopodin. Original magnification, ×400 (A and B, top); ×1,000 (B, bottom).