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. 2012 Jan 17;122(2):674–692. doi: 10.1172/JCI60070

Figure 2. Clustered, activated CD16/7-nephrinCD and CD16/7-Neph1CD induce lamellipodia formation in cultured human podocytes.

Figure 2

(A) CD16/7-nephrinCD or CD16/7-Neph1CD was coexpressed with actin-GFP in human podocytes, which were activated by clustering as in Figure 1. Transfected cells with structures typical of lamellipodia on more than 30% of their circumferences were counted as cells with lamellipodial protrusions. (B) Using the same system as in A, cells were treated with PP2 or PP3 before activation. Transfected cells with actin tails longer than 5 μm or lamellipodia extending over more than 30% of the cell circumference were counted positive for these features. 20 transfected cells total were counted per experiment. Data (mean ± SEM) are from single experiments and are representative of 3 unique experiments.