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Cas-null (A), Cas-WT (B), and Cas-15F (C) MEFs were transfected with CD16/7-nephrinCD and Crk-myc as indicated. Cells were activated by clustering as in Figure 1 and analyzed by confocal microscopy. Crk-myc was stained with myc antibody and labeled with secondary antibody (Alexa Fluor 488). Cells treated with PP2 prior to clustering are also shown. (A–C) Original magnification, ×630. yz plane reconstructions are shown at far right.
