Cytotoxicity of virus against 6 human gastroesophageal carcinoma cell lines in vitro. All cell lines were susceptible to viral oncolysis. The (A) MKN-74, (B) MKN-1, and (C) BE-3 cell lines required higher multiplicities of infection (MOI) to achieve cytotoxicity. MOI refers to the ratio of viral plaque-forming units relative to number of tumor cells. Open triangle, MOI = 1.0; square, MOI = 0.1; and diamond, MOI = 0.01. The (D) OCUM-2MD3, (E) MKN-45, and (F) AGS cell lines were susceptible to viral oncolysis at low MOI levels. Square, MOI = 0.1; diamond, MOI = 0.01; open circle, MOI = 0.001.