Table 3. Geometry of the most important ligand⋯NodZ polar interactions.

Interaction		Distance (Å)
Ligand	NodZ	3siw†	3six†
N1	Asp270 Oδ2	2.78	2.97
N2	Asp270 Oδ2	3.18	—
N7	His175 N∊2	2.83	2.79
O2′	Tyr45 Oη	2.59	—
O3′	Tyr45 Oη	2.87	3.27
O1α	Gly16 N	3.10	—
O2α	Asp17 N	3.12	—
O1β	Ala288 N	2.87	—
O2β	Ser287 Oγ	2.61	—
O2β	Arg177 Nη1	3.09	—
O3β	Arg177 Nη1	2.91	—
N7	Arg177 Nη1	—	3.09‡

^† 3siw corresponds to the NodZ–GDP complex and 3six to the NodZ–GDP-Fuc complex.

^‡Plane-to-plane stacking interaction between the π-electron system of the purine ring and the positively charged guanidinium group of the arginine side chain.