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. Author manuscript; available in PMC: 2012 Jan 26.
Published in final edited form as: Cell. 2006 Sep 8;126(5):917–928. doi: 10.1016/j.cell.2006.06.053

Figure 5. SpoIIQ and SpoIIIAH Contribute to Engulfment in Intact Cells.

Figure 5

(A–L) Samples were harvested at t3 and a fusion assay was performed to assess the completion of engulfment. FM 4–64 is membrane impermeable (red); Mitotracker Green is permeable (green; MTG shown in overlay). Fused sporangia, indicated by arrows, have completed engulfment; the unfused sporangia, indicated by arrowheads, have not. Percent of sporangia fused at t3 (A–G) or percent fused relative to ΔspoIIQ∷spc, amyE∷spoIIQΩcat, spoIIB∷erm (KP4369) at t3.5 (H–L) is shown (>200 sporangia scored for each strain). (A) Wild-type (PY79), (B) ΔspoIIQ∷spc (KP575), (C) ΔspoIIIAH (MO1429), (D) ΔspoIIIAG-HΩkan (KP10359), (E) spoIID31 (KP38), (F) spoIID31, ΔspoIIQ∷spc (KP4270), (G) spoIID31, ΔspoIIIAH (KP4296), (H) ΔspoIIQ∷spc, amyE∷spoIIQ, spoIIB∷erm (KP4369), (I) ΔspoIIQ∷spc, spoIIB∷erm (KP4276), (J) ΔspoIIQ∷spc, amyE∷spoIIQ(rbm9), spoIIB∷erm (KP4373), (K) ΔspoIIQ∷spc, amyE∷spoIIQ(rbm10), spoIIB∷erm (KP4377), (L) ΔspoIIQ∷spc, amyE∷spoIIQ(rbm13), spoIIB∷erm (KP4370). (M) Cartoon of membrane fusion assay showing FM 4–64 (red) and Mitotracker Green (green).