Skip to main content
. 2012 Jan 26;8(1):e1002470. doi: 10.1371/journal.pgen.1002470

Figure 4. In vitro transcriptional regulation of human CSA and HR23A by USF.

Figure 4

(A) Schematic representation of the CSA promoter-luciferase constructs. The construct contains the sequence from −847 to +1 of the CSA promoter linked to the luciferase reporter. E-box is represented in dark gray and its position is indicated on top. Cross shows mutated E-box. (B) CSA promoter-luciferase activity measured after co-transfection of XB2 keratinocytes with WT or mutated CSA promoter-luciferase constructs with pCMV-USF-1, pCMV-USF-2 expression vectors or pCMV empty vector (control). (C) CSA promoter-luciferase activity measured 30 min or 5 h after UV induction (6×10 J/m2) of XB2 cells transfected with WT or mutated CSA promoter-luciferase constructs. (D) Schematic representation of the HR23A promoter-luciferase constructs. The construct contains the sequences from −186 to +73 of the HR23A promoter linked to the luciferase reporter. E-boxes are represented in dark gray, GC-box in light gray, and positions are indicated on top. Crosses show mutated boxes. (E) HR23A promoter-luciferase activity measured after co-transfection of XB2 keratinocytes with pCMV-USF-1 or pCMV-USF-2 expression vectors or empty vector. (F) WT and mutated HR23A promoter-luciferase activities in XB2 cells following UV-irradiation (6×10 J/m2). (G) WT and mutated HR23A promoter-luciferase activities transfected in XB2 cells with pCMV-USF-1 expression vector and UV irradiated (6×10 J/m2). Error bars indicate s.e.m.; n = 3; one asterisk, P<0.05, two asterisks, P<0.01, three asterisks, P<0.001.