Table 1. Sperm motility and kinematics after computer assisted sperm analysis (CASA) of stallion spermatozoa stored during five days (day 1 D1 to day 5 D5) at 5°C FE fresh extended sperm, CC sperm processed through colloidal centrifugation.
| D1 | D2 | D3 | D4 | D5 | ||||||
| FE | CC | FE | CC | FE | CC | FE | CC | FE | CC | |
| TM% | 84.2±11.1a | 88.7±8.5 a | 65.4±14.1e | 72.9±18.6 e | 60.9±16.5b,e | 57.8±23.5b,e | 48.6±17.3 b | 47.1±26.9 b | 41.7±20.6c | 37.4±21.2c |
| LM% | 55.7±11.9a | 66.0±12.5a | 38.1±15.9e | 49.2±22.5 e | 29.9±11.3c | 29.1±21.5c | 20.1±11.9d | 19.8±16.2d | 14.4±7.3d | 10.1±9.9d |
| RS% | 34.9±15.1a | 44.9±19.7a | 14.2±8.7e | 22.7±15.0e | 10.9±8.0ed | 13.5±14.8ed | 6.5±4.06d | 9.1±8.6d | 6.7±5.8d | 4.7±5.5d |
| VCLµm/s | 83.2±18.4a | 90.8±19.5a | 62.3±15.2e | 68.8±22.1e | 58.4±15.0ed | 57.2±17.4ed | 53.7±13.4d | 50.9±19.2d | 53.5±14.1d | 44.5±20.1d |
| VSLµm/s | 34.1±9.0a | 44.9±11.4a | 25.5±10.2e | 32.8±12.5e | 21.2±6.9c | 22.1±10.4c | 18.1±5.7cd | 17.5±8.7cd | 15.0±3.9d | 14.1±8.1d |
| VAPµm/s | 48.9±14.4a | 60.9±17.0a | 34.2±11.4e | 41.9±14.5 e | 29.7±8.1c | 30.1±11.9c | 26.3±6.1c | 25.8±9.6c | 24.0±5.6c | 21.7±9.6c |
| ALHµm | 3.7±0.6a | 3.6±0.8a | 3.4±0.5a | 3.2±0.5a | 3.5±0.4a | 3.4±0.4a | 3.7±0.3a | 3.4±0.8b | 4.3±0.4b | 3.3±1.31a |
| BCF Hz | 11.1±2.1a | 10.7±1.7a | 10.4±2.1a | 11.5±1.7a | 10.3±2.1a | 11.1±1.9a | 9.6±2.1ab | 9.4±3.7ab | 8.4±1.1b | 8.7 ±3.7b |
TM% total motile sperm, LM% linear motile sperm, RS% rapid sperm, VCL circular velocity, VSL straight line velocity, VAP average velocity, ALH lateral head displacement, BCF beat cross frequency. Within a row values with different superscripts differ statistically a-e, P<0.01. (means ± SD) Results are derived from 28 identical experiments (7 stallions, 4 ejaculates per stallion).