Table 2. Sperm membrane integrity (SYBR-14/PI) of stallion spermatozoa stored during five days (day 1 D1 to day 5 D5) at 5°C FE fresh extended sperm, CC sperm processed through colloidal centrifugation.
D1 | D2 | D3 | D4 | D5 | ||||||
FE | CC | FE | CC | FE | CC | FE | CC | FE | CC | |
LIVE % | 78.7±13.1a | 84.7±11.3b | 82.9±7.7a | 88.8±6.7b | 73.8±13.1a | 81.6±5.3b | 74.0±9.17a | 80.3±11.0b | 72.1±7.7a | 74.4±14.0a |
DEAD% | 16.3±10.3 | 10.0± 8.1 | 12.5±7.3 | 6.6±7.0 | 17.9±11.9 | 11.5±6.1 | 17.5±7.2 | 12.2±9.5 | 19.0±8.8 | 17.9±13.1 |
DAMAGED% | 5.0±5.9 | 5.2±9.5 | 4.5±2.8 | 3.9±5.6 | 8.2±7.7 | 6.9±6.7 | 8.5±8.9 | 7.4±8.9 | 8.8± 7.7 | 7.6±6.5 |
LIVE % (SYBR-14+ sperm), DEAD% (PI+ sperm), DAMAGED, (SYBR-14+/PI+sperm). Within a row values with different superscript differ statistically a-b p<0.01. (means ± SD) Results are derived from 28 identical experiments (7 stallions, 4 ejaculates per stallion).