Table 3. Membrane intactness and subtle changes in sperm membrane integrity of stallion spermatozoa stored during five days (day 1 D1 to day 5 D5) at 5°C FE fresh extended sperm, CC sperm processed through colloidal centrifugation.
D1 | D3 | D5 | ||||
FE | CC | FE | CC | FE | CC | |
Intact% | 58.1±12.9c | 72.4±13.2a | 51.3±9.9c | 60.4±9.1c | 45.8±11.7b | 49.9±11.0b |
YoPro+ % | 12.7±6.7a | 8.6±2.9a | 9.4±2.7a | 8.41±3.3a | 20.7±18.9b | 18.5±10.8b |
YoPro+/Eth+ % | 21.6±12.9 | 12.3±11.1 | 28.2±9.9 | 18.5±3.7 | 22.9±13.1 | 25.9±9.9 |
Eth+ % | 7.5±6.2 | 6.6±7.0 | 11.0±8.6 | 12.8±6.2 | 10.5±10.3 | 5.5±4.6 |
Intact spermartozoa are those not stained and thus represent spermatozoa with completely intact membranes. YoPro+ are early apoptotic sperm depicting an increase in sperm membrane permeability, YoPro+/Eth+ are late apoptotic and Eth+ are necrotic spermatozoa. Within a row values with different superscript differ statistically a-c p<0.01. (means ± SD) Results are derived from 28 identical experiments (7 stallions, 4 ejaculates per stallion).