Table 4. Active caspases 3 and 7 of stallion spermatozoa stored during five days (day 1 D1 to day 5 D5) at 5°C FE fresh extended sperm, CC sperm processed through colloidal centrifugation.
D1 | D3 | D5 | ||||
FE | CC | FE | CC | FE | CC | |
High activity% | 27.9±31.8 | 28.3±32.6 | 18.9±15.9 | 18.2±25.3 | 30.2±28.9 | 28.3±22.9 |
Low activity% | 43.7±27.7 | 48.6±35.3 | 37.9±15.0 | 42.61±9.7 | 34.6±30.7 | 34.6±24.4 |
Dead sperm% | 28.6±17.7a | 23.3±19.7a | 43.5±4.5b | 40.8±8.8b | 35.4±18.8ab | 37.5±10.6ab |
High activity are spermatozoa with high caspase 3 an 7 activity, low activity are spermatozoa with low caspase 3 and 7 activity and dead sperm correspond to necrotic (Ethidium positive spermatozoa). Within a row values with different superscript differ statistically, a-b p<0.01. (means ± SD) Results are derived from 28 identical experiments (7 stallions, 4 ejaculates per stallion).