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. 2012 Jan 26;7(1):e30688. doi: 10.1371/journal.pone.0030688

Table 4. Active caspases 3 and 7 of stallion spermatozoa stored during five days (day 1 D1 to day 5 D5) at 5°C FE fresh extended sperm, CC sperm processed through colloidal centrifugation.

D1 D3 D5
FE CC FE CC FE CC
High activity% 27.9±31.8 28.3±32.6 18.9±15.9 18.2±25.3 30.2±28.9 28.3±22.9
Low activity% 43.7±27.7 48.6±35.3 37.9±15.0 42.61±9.7 34.6±30.7 34.6±24.4
Dead sperm% 28.6±17.7a 23.3±19.7a 43.5±4.5b 40.8±8.8b 35.4±18.8ab 37.5±10.6ab

High activity are spermatozoa with high caspase 3 an 7 activity, low activity are spermatozoa with low caspase 3 and 7 activity and dead sperm correspond to necrotic (Ethidium positive spermatozoa). Within a row values with different superscript differ statistically, a-b p<0.01. (means ± SD) Results are derived from 28 identical experiments (7 stallions, 4 ejaculates per stallion).