Table 5. Annexin-V assay of stallion spermatozoa stored during five days (day 1 D1 to day 5 D5) at 5°C FE fresh extended sperm, CC sperm processed through colloidal centrifugation.
| D1 | D3 | D5 | ||||
| FE | CC | FE | CC | FE | CC | |
| Live (A-PI-)% | 71.8±15.7a | 69.5±18.2a | 47.9±10.6b | 53.6±12.6b | 40.0±15.4b | 48.9±10.2b |
| A+% | 6.1±4.3 | 14.2±15.7 | 8.5±10.0 | 8.5±10.0 | 15.8±15.8 | 10.3±9.0 |
| A+PI+% | 1.0±1.5a | 0.8±1.1a | 2.2±2.0 | 2.8±2.3 | 2.9±3.9b | 2.9±3.3b |
| A-PI+% | 20.9±14.8a | 15.6±11.3a | 41.4±9.2b | 31.2±15.9b | 41.2±12.9b | 37.9±12.8b |
Live % percentage of live sperm, A+, annexin positive sperm, spermatozoa depicting translocation of PS, A+/PI dead spermatozoa depicting PS translocation, A-PI+ necrotic spermatozoa Within a row values with different superscript differ statistically a-b p<0.01. (Means ± SD) Results are derived from 28 identical experiments (7 stallions, 4 ejaculates per stallion).