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. 2012 Jan 26;7(1):e30688. doi: 10.1371/journal.pone.0030688

Table 6. Mitochondrial membrane potential (Δϕm) and lipid peroxidation (LPO) of stallion spermatozoa stored during five days (day 1 D1 to day 5 D5) at 5°C FE fresh extended sperm, CC sperm processed through colloidal centrifugation.

D1 D3 D5
FE CC FE CC FE CC
High % 4.4±8.8 8.3±12.2 4.4±7.4 5.6±6.2 2.2±4.4 6.2±5.8
High and low% 27.3±16.3 23.2±23.6 23.6±17.3 30.4±22.2 31.4±13.5 10.9±16.8
Low% 68.1±22.5 67.8±33.3 71.9±22.5 63.9±26.9 62.2±17.7 82.9±18.4
LPO% 2.0± 2.4 3.4±5.1 5.0± 5.8 4.1±4.2 3.1±3.1 1.8±1.6

(Means ± SD) Results are derived from 28 identical experiments (7 stallions, 4 ejaculates per stallion). High spermatozoa depicting high Δϕm, High and Low spermatozoa depicting simultaneously mitochondria with low and high Δϕm, Low spermatozoa with low Δϕm, LPO spermatozoa showing peroxidation of the lipids of their membranes.