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. 2012 Jan 26;7(1):e30472. doi: 10.1371/journal.pone.0030472

Figure 8. Overexpression of sim3 reversed the temperature sensitive growth in the asf1-33 mutant.

Figure 8

(A) Cultures of L972 (asf1+), SKP605-33 (asf1-33-13myc-kanr), and SKP605-33 harboring pREP41 or pREP41-sim3 were serially diluted with sterilized water and spotted on YES plates. Plates were incubated at 26, 34 and 36°C. (B) Cell morphology of the cells used in (A) was observed microscopically.