Figure 3. Proinflammatory cytokines (IL-1β, IL-6, and TNF-α) produced by BV2 cells treated with PrP_106–126 in the presence or not of anti-CD36 antibody.

Cells were first pre-incubated or not with Anti-CD36 antibody (1 µg/ml), and then treated for 12 h with PBS or PrP_106–126 (50 µM). The level of released cytokines was determined in culture supernatants by ELISA as described in Materials and methods. The amount of cytokines (A: IL-1β, B: IL-6 and C: TNF-α) is expressed as ng/l cell supernatant. All data are means ± s.d. of triplicate samples and are representative of an experimental n of 3 or 4, * P<0.05.