Figure 5. Western blot analysis of p65 nuclear translocation.

BV2 microglia were treated or not with two different concentrations of neurotoxic prion peptides (50 µM and 100 µM, respectively) for 24 h. The role of CD36 in NF-KB activation was examined by 1 h-preincubation with anti-CD36 antibody. A. Extracts were prepared and immunoblotted with p65 antibody as described in Materials and Methods. The blot was stripped and reprobed with anti-Max antibody to estimate the total amount of nuclear protein loaded in gel. Representative blots of p65 and Max are shown. B. Bars represent the relative levels of p65, compared with Max, and were expressed as arbitrary units. Data are the means±S.D of three independent experiments. * P<0.05, significantly different from control cells.