Figure 7. Western blot analysis of p-Fyn.

BV2 microglia were treated or not with neurotoxic prion peptides (100 µM) for 24 h after 1 h-preincubation with anti-CD36 mAb. The role of Fyn in CD36 signaling was examined by assessing Fyn phosphorylation. A. Extracts were prepared and immunoblotted with phospho-Fyn (p-Fyn) antibody as described in Materials and Methods. The blot was stripped and reprobed with anti-β-actin antibody to estimate the total amount of protein loaded in gel. Representative blots of p-Fyn and β-actin are shown. B. Bars represent the relative levels of cleaved p-Fyn, compared with β-actin, and were expressed as arbitrary units. Data are the means±S.D of three independent experiments. * P<0.05, significantly different from control cells.