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. 2012 Jan 26;8(1):e1002500. doi: 10.1371/journal.ppat.1002500

Figure 3. Expression of Salmonella SPI-1 and SPI-2 effector proteins in ΔmreC.

Figure 3

(A) Expression of SPI-1 proteins in WT SL1344, ΔmreC, ΔSPI-1, and ΔSPI-2 mutants during SPI-1 inducing conditions as revealed by western blotting with polyclonal αSipA and αSipC antibodies. Expression of SPI-2 in WT SL1344, ΔmreC, ΔSPI-1, and ΔSPI-2 mutants during SPI-2 inducing conditions as revealed by western blotting of membrane fraction samples with polyclonal αSseB antibody. Samples representing total proteins and secreted proteins are shown. Arrows indicate the respective protein bands. (B)Transcriptional expression profiles of hilA, hilC, hilD, sopB (SPI-1) and ssaG (SPI-2) promoter reporters in WT SL1344 (blue diamonds) and ΔmreC (red squares). Experiments were repeated at least three times and error bars indicate standard deviation.