Fig. (4). A–B: Representative blocking zymogram for conditioned media from primary cell cultures.
Three primary cell culture specimens are shown (1, 6 and 7). EDTA had a significant inhibiting effect on the gelatinase activity of MMP-2 and MMP-9 in the control (CD-27SK) and the GCT stromal cell cultures. [a=activated, z=zymogen].