Skip to main content
. Author manuscript; available in PMC: 2012 Dec 28.
Published in final edited form as: Oncogene. 2011 Oct 17;31(26):3164–3176. doi: 10.1038/onc.2011.480

Figure 8. Depletion of Tregs in IKTA mice increases infiltration of lungs with CD8 lymphocytes and reduces the number of atypical adenomatous hyperplasia (AAH) lesions.

Figure 8

A) Photomicrographs of lung sections demonstrating AAH lesions at 6 weeks post-urethane in IKTA mice treated with anti-CD25 antibodies (CD25) or isotype control antibodies (IgG) per protocol (original magnification x200). B–D) Percentages of total CD4+ T cells, CD8+ T cells, and CD4+ Foxp3+ Tregs identified by flow cytometry in lungs from anti-CD25 or isotype control antibody-treated IKTA mice at 6 weeks after injection of urethane (1 week after last antibody dose) (n=4 per group, *=p<0.05). E,F) Percentages of CD4+Foxp3-IL-4+ and CD4+Foxp3-IFNγ+ lymphocytes isolated from lungs of doxycycline-treated IKTA mice at 1 week after a single IP dose of 500 μg of anti-CD25 (PC61) or isotype control antibodies. Cells were re-stimulated with PMA/ionomycin for 6 hours in vitro and analyzed by flow cytometry.