Figure 2. Cotransplantation with MDSC protects islet allografs.
(A) Impact of cotransplantation with MDSC on survival of islet allografts. 300 islets (BALB/c) mixed with 2.5 × 106 MDSC generated by B6, BALB/c or C3H HpSC were transplanted into diabetic B6 recipients (islet allografting alone and cotransplanted with B6 HpSC or B6 DC served as controls). Cotransplantation with HpSC or DC (both from B6), and transplantation of islet allografts alone served as controls. (B) Randomly selected 3 recipients with euglycemia for >60 days (normal B6 mice served as controls) underwent glucose tolerance test as described in Methods, and the data were demonstrated as mean glucose (mg/dl) ±1SD. (C) The kidney bearing islet allografts was removed in a recipient with euglycemia for 125 days, resulting in prompt hyperglycemia. The islet grafts were stained for insulin (red). (D)