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Analogous Mutations in the SNARE Motifs of VAMP2, VAMP3, and VAMP8 Abolish Their Endocytosis, Related to Figure 3
Endocytosis of anti-HA in cells expressing HA-tagged VAMPs, using the flow cytometry assay described in Figure S1B. The wild-type constructs were efficiently internalized, but the VAMP2-HAV43AM46A mutant, the VAMP3-HAV30AM33A mutant, and the VAMP8-HAK24AM27A mutant remained on the plasma membrane.
