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Localization of Anti-HA in Cells Expressing Different VAMP8-HA Proline Mutants, Related to Figure 7
Control and siRNA-treated cells were mixed together and allowed to endocytose the antibody for 40 min, then processed for immunofluorescence. Knocking down CALM prevents antibody uptake in cells expressing the L44PL51P mutant, but in cells expressing the L16PL23P mutant the antibody stays on the cell surface whether or not CALM is knocked down. The scale bar represents 20 μm.
