Fig. (2).

Re-engineering meganuclease specificity: example of a custom meganuclease targeting a sequence from the human XPC gene. (a) Comparison of the starting and final targets. (b) Crystal structure of the engineered I-CreI variant in complex with target DNA. Details of protein-DNA contacts from the encircled region are highlighted (c) to demonstrate how a clustered approach leads to significant changes in specificity contacts. The native protein-DNA contacts for I-CreI (d) are shown for reference. Adapted from [161].