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. 1981 Mar 11;9(5):1069–1086. doi: 10.1093/nar/9.5.1069

Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.

I Bozzoni, E Beccari, Z X Luo, F Amaldi
PMCID: PMC326737  PMID: 6112733

Abstract

Poly-A+ mRNA from Xenopus laevis oocytes, partially enriched for r-protein coding capacity has been used as starting material for preparing a cDNA bank in plasmid pBR322. The clones containing sequences specific for r-proteins have been selected by translation of the complementary mRNAs. Clones for six different r-proteins have been identified and utilized as probes for studying their genomic organization. Two gene copies per haploid genome were found for r-proteins L1, L14, S19, and four-five for protein S1, S8 and L32. Moreover a population polymorphism has been observed for the genomic regions containing sequences for r-protein S1, S8 and L14.

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