Fig. 4.

Both Lec1 and CHO cells show comparable high levels of virus binding. CHO-K1 (red line) and Lec1 (black line) cells were exposed to rZJ1 at an MOI of 50 or 10 (A and B) on ice to allow virus binding. After 90-min incubation on ice, cells were incubated with the monoclonal antibody to NDV HN protein (6B1) for 45 min at 4°C, and then incubated with the FITC-labeled antibody for 45 min at 4°C. Virus binding was analyzed by flow cytometry. Cells analyzed in the absence of monoclonal antibody to NDV HN protein are shown in the filled light-gray trace.