Fig. 7.

Effect of sialidase treatment on NDV infection and fusion in Lec1 cells. Lec1 cells were incubated in the presence of 200 mU/mL sialidase from V. cholerae for 3 h at 37°C prior to infection with NDV (rZJ1-GFP or rLX-RFP) at an MOI of 1. (A) At 16 hpi, infected cells were visualized for GFP expression under the inverted fluorescence microscope. (B) At 16 hpi, sialidase-treated (red line) and non-treated (black line) Lec1 cells were also subjected to flow cytometric analysis for quantitating NDV infection (rLX-RFP). Mock-infected Lec1 cells were shown in the filled light gray trace.