Skip to main content
. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Immunol. 2011 Dec 30;188(3):1479–1490. doi: 10.4049/jimmunol.1102253

Fig 1. Expression of SWAP-70 by murine and human eosinophils.

Fig 1

(A) Western blot of human eosinophil lysates (two representative allergic donors) as well as lysates of BMMC and eosinophils from WT and Swap-70−/− mice with affinity purified polyclonal antibodies against murine SWAP-70. Lysates of Swap-70−/− and WT BMMC served as the negative and positive control, respectively. (B) RT-PCR with total RNA from human eosinophils (two representative allergic donors) as well as eosinophils from WT and Swap-70−/−mice using species specific primers for the first exon of the SWAP-70 gene. Total RNA from WT BMMC was used as the positive control. Expression of GAPDH (for human eosinophils) and β-actin (for murine BMMC and eosinophils) served as internal controls. (C) Immunofluorescence staining for eosinophil-specific MBP and SWAP-70 expression by WT and Swap-70−/− eosinophils by confocal microscopy with rat anti-mouse MBP antibodies and affinity purified rabbit anti-mouse SWAP-70 antibodies followed by FITC-conjugated goat anti-rat or anti-rabbit IgG, respectively. Rat IgG1 (for MBP) and rabbit IgG (for SWAP-70) were used as isotype controls. Data shown in C is representative of three independent experiments with eosinophils from three different mice for each group.