Figure 1.
The sugar composition of the cell culture medium mediated the binding of Calcein fluorescent probe-labeled MDA-MB-468 cells to HUVECs, as measured by mean fluorescence intensity (MFI). Based on three independent replicated assays, cells cultured in glucose, glucose: fructose, and fructose showed MFI values of 2321 (±120), 2707 (±358) and 3367 (±133), respectively. Block ANOVA (blocking on assay replication) analysis showed a statistically significant difference in the MFI values among the carbon sources (F2,4 =10.73, p=0.025), with the largest difference found between cells cultured in glucose versus fructose (Tukey-adjusted p=0.022).