Table 2.
Location, orientation, hydration, and dynamics of DiI
| Rotational relaxation |
||||||
|---|---|---|---|---|---|---|
| α/nm2 | DiI locationa/nm | DiI orientationc/degrees | MSD at τ = 200 ps/Å2 | τ1/ns | τ2/ns | Hydration (molecules) |
| Standard error → | ±0.01 | ±0.20 | ±0.06 | ±0.03d | ±0.25d | — |
| 0.635 | 0.62 | 4.74 (56.24) | 3.73 | 1.09 | 9.69 | 4.7 |
| 0.650 | 0.54 | 5.5b | — b | — b | — b | 5.4 |
| 0.675 | 0.57 | 2.14 (54.88) | 4.34 | 0.95 | 8.07 | 4.7 |
| 0.687 | 0.62 | −2.56 (55.14) | 4.65 | 0.75 | 7.56 | 4.8 |
| 0.700 | 0.60 | 6.05 (56.71) | 4.46 | 1.13 | 7.09 | 4.9 |
| 0.725 | 0.53 | 3.22 (61.08) | 5.19 | 0.89 | 6.14 | 5.6 |
| 0.750 | 0.54 | 2.07 (52.90) | 5.49 | 0.82 | 7.19 | 5.1 |
a
Positive values indicate DiI location below the lipid–water interface.
b
A bimodal distribution of dye orientation was observed, with one dye molecule temporarily trapped in a metastable configuration with an average orientation of around 60 degrees. For this reason, the lateral and rotational dynamics are not reported.
c
The values in parenthesis indicate full width half maximum (FWHM) of the distribution.
d
95% confidence intervals obtained from curve-fitting.