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Summary of Tm for the MG aptamer
| [MG aptamer] (μM) | Tm (°C) | [MG aptamer] (μM) | Tm (°C) |
|---|---|---|---|
| in 0 mM Mg2+ | in 10 mM Mg2+ | ||
| 1.3 | 59.7 ± 0.3 | ||
| 2.5 | 58.5 ± 0.5 | 2.5 | 85.2 ± 0.3 |
| 5 | 57 ± 1 | 5 | 85.3 ± 0.8 |
| 10 | 58 ± 1 | 10 | 85 ± 1 |
| 20 | 57 ± 1 | 18 | 84.7 ± 0.3 |
All experiments are an average of 3 scans.
