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. Author manuscript; available in PMC: 2013 Jan 10.
Published in final edited form as: Biochemistry. 2011 Dec 16;51(1):565–572. doi: 10.1021/bi201642p

Table 4.

ITC data for MG binding as a function of MgCl2 concentration.

[MgCl2] (mM) Apparent Stoichiometry Apparent Kd (nM) ΔH° (kcal/mol)
0 0.59 1600 -26
0.1 0.60 130 -40.
0.5 0.55 93 -42
1 0.55 150 -48
10 0.41 ± 0.02 100 ± 20 -55 ± 4

All experiments were performed in 10 mM sodium cacodylate (pH 5.8) with 10 mM KCl. Experiments were at 30 °C, which was chosen because it is in the center of the lower temperature regime.