Figure 4. Growth rate of the conditional mutant at different concentrations of CdCl₂.

(A) Growth curves of strains COL (black diamond), and the COLpCadmurT-gatD conditional mutant in TSB supplemented with (black triangle) 0 µM of CdCl₂ and (white square) 0.2 µM of CdCl₂. (B) Growth on solid medium with or without supplementation of 0.2 µM CdCl₂ of COLpCadmurT-gatD. (C) Growth curves in liquid medium supplemented with Cm (10 µg/ml) of the complementation mutants COLpCadmurT-gatD+pSKmurT with 0.2 µM CdCl₂ (white circle) or without CdCl₂ (black circle), COLpCadmurTgatD+pSKgatD with 0.2 µM CdCl₂ (white triangle) or without CdCl₂ (black triangle), and the control strain COLpCadmurT-gatD+pSK with 0.2 µM CdCl₂ (white line) or without CdCl₂ (black line). (D) Growth on solid medium, with or without supplementation of 0.2 µM CdCl₂, of the complementation strains COLpCadmurT-gatD+pSKmurT, COLpCadmurT-gatD+pSKgatD, and the control strain with the plasmid pSK5632, COLpCadmurT-gatD+pSK.