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. 2011 May 14;18(Pt 4):630–636. doi: 10.1107/S0909049511011836

Figure 1.

Figure 1

DNA double-strand break repair in V79 cells after MRT pattern emulation. Fluorescent staining with the γH2AX antibody (a marker of DNA damage) shows synchrotron MRT emulation in cell culture using a slit system of varying beam widths and interbeam spacings. The in-air entrance dose was 10 Gy in all cases and the cells (V79 Chinese hamster lung cells) were fixed 1 h (a–e) and 12 h (f–j) post-irradiation. (a, f) 25 µm beam width with centre-to-centre spacings of between 50 and 300 µm, (b, g) 50 µm beam width with spacings of between 75 and 300 µm, (c, h) 100 µm beam width with a 200 µm centre-to-centre spacing, and (d, i) 150 µm beam width and a 200 µm spacing. (e, j) Zoomed images from 100 µm beam width with a 200 µm centre-to-centre spacing. Bars indicate 100 µm.